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Figure 2 | Retrovirology

Figure 2

From: Induction of the HIV-1 Tat co-factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs

Figure 2

shRNA against cyclin T1 expressed from a lentiviral vector can efficiently knock down cyclin T1 protein expression. (A) Untransduced cells (Parental MM6 cells) or MM6 cells infected at a m.o.i. of five with lentiviral vectors expressing a shRNA against cyclin T1(shRNA-T1) or a control shRNA against a mismatch sequence in cyclin T1 (shRNA-Con) were analyzed by flow cytometry at day five post-infection. The lentiviral vectors express an eGFP marker protein. The percentages of the GFP positive cells are indicated. (B) Cell extracts were prepared at day five post-infection from the cultures described in A which were either untreated or treated with PMA for 24 hours. Immunoblots were performed to measure levels of cyclin T1, CDK9 and β-actin proteins.

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Retrovirology

ISSN: 1742-4690

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