Figure 1

Immunoblot analysis of CD4 dimerization levels of CD4-WT and mutants. HEK 293 cells were transfected with SVCMV CD4-Q344E (lane 2), SVCMV CD4-WT (lane 3) and SVCMV CD4-E91K, E92K (lane 4) by a calcium phosphate method. Cells were lysed 48 hours post-transfection. Equivalent amounts of cell lysates were separated by SDS-PAGE, in a non-reducing Laemmli buffer and transferred onto a nitrocellulose membrane. Immunoblot analysis was performed using a CD4-specific polyclonal Ab. The lower panel represents a shorter exposition of the same membrane, showing unsaturated bands of CD4 monomers. This is a representative result of 5 independent experiments. (UT) represent the untransfected negative control.