Functional analysis of human T lymphotropic virus type 2 Tax proteins

Retrovirology

Table 1 Transactivation phenotypes of previously characterised Tax mutants in Jurkat cells

Jurkat cells were co-transfected with 250 ng of the indicated Tax 2 plasmids, 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs, the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer. Firefly reporter activities were normalised using Renilla luciferase values. Normalised values for mutant Tax proteins were calculated as a percentage of the wildtype Mo or Tax 2B values which was set at 100%.

ISSN: 1742-4690