Figure 4

Expression levels of Tax 2 wildtype and mutant proteins. 293T cells were transfected with either wildtype or mutant Tax plasmids and cell lysates were subjected to electrophoresis on 10% SDS polyacrylamide gels. Western blots were performed using anti-HIS to detect Tax expression and anti-tubulin to detect Tubulin which was used as a loading control. Each panel shows the expression levels of both wildtype (WT) and corresponding mutant proteins for the indicated Tax proteins.