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Figure 2 | Retrovirology

Figure 2

From: Factor correction as a tool to eliminate between-session variation in replicate experiments: application to molecular biology and retrovirology

Figure 2

Comparison of normalisation, standardisation and factor correction. Mean (and SEM) of the data of the molecular-biology data set from Figure 1 A: original data. B: normalised data. C: standardised data. D: data after factor correction. Note that normalisation, standardisation, and factor correction reduce the variation within each condition. However, normalisation (B) leads to loss of variance in the control condition ('c') and to added variation in the other conditions. Standardisation (C) of this incomplete data set leads to increased variation, compared to factor correction, in some conditions. With factor correction (D) all conditions retain their statistical variance, which is generally smaller than after normalisation and standardisation. An asterisk indicates a statistically significant difference between the DNA construct and construct 1 (t-test; P < 0.05). Note that the number of observations per construct in these comparisons ranges from 2 to 5.

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Retrovirology

ISSN: 1742-4690

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