Coordinate enhancement of transgene transcription and translation in a lentiviral vector

Retrovirology

Table 2 The combination of PCE and CMV IE increased Luc activity in transduced cells

	Luc activity (Relative Light Units)^a
	Replicate Experiment
Vector	HeLa	CEM-A	D17	293
U3-Luc	13,639 ± 2,150 (1)^b	17,100 ± 524 (1)	18,093 ± 349 (1)	15,099 ± 539 (1)
PCE-Luc	51,998 ± 3108 (3.8)*	52,372 ± 3,354 (3.0)*	50,412 ± 2,997 (2.7)*	27,294 ± 1,173 (1.8)*
IE-U3-Luc	ND^c	ND	ND	26,197 ± 95 (1.7)
IE-PCE-Luc	ND	ND	ND	59,620 ± 286 (3.9)

^a Equivalent vector virus particles were measured by Gag p24 ELISA and 4 × 10⁵ pg Gag was used to transduce the indicated target cell lines. Total cellular protein was harvested 48 hrs post-transduction and equivalent protein was subject to Luciferase assay. Standard deviations were calculated from results of two or more replicate experiments. ANOVA with repeated measures determined that increases in response to PCE were significant, (p-value of 0.017).
^b (), Fold difference relative to U3-luc vector.

ISSN: 1742-4690