Figure 1
From: Coordinate enhancement of transgene transcription and translation in a lentiviral vector
Genomic structure of self-inactivating lentiviral vectors that lack or contain PCE translation enhancer. HIV-1 based lentiviral vectors were derived from pHR' [36]. Black rectangles represent HIV-1 long terminal repeats; PBS, primer binding site; Ψ, the core packaging signal; extended packaging signal that corresponds to 350 nt HIV-1 gag open reading frame; 5'ss and 3'ss, splice sites; RRE, Rev responsive element; PPT, polypurine tract; Δ indicates deletion of HIV-1 promoter sequences between -418 to -18; white boxes represent SNV sequences; Prom corresponds to spleen necrosis virus (SNV) U3 promoter; PCE is the 165 nt RU5 region of SNV; CMV IE, cytomegalovirus immediate early enhancer.