Figure 6
From: Intracellular assembly and budding of the Murine Leukemia Virus in infected cells
Infectivity of intracellular particles released by freeze-thaw and sonication treatment. FIA was used to quantitate infectious particles present in the cell lyzed by freeze-thaw and sonication (cell lysate) or in the last wash of cells left intact (control). A) One typical FFU labeled with anti-Env antibody and detected in FIA. Insert: magnification of the boxed area showing infected (arrow) and non infected (arrowhead) Dunni cells. B) Results of the FIA expressed as the total number of infectious FFU detected in the total lysate of 5 × 106 cells. Lysis and infectivity experiments were performed at least 3 times and each infection test was performed in triplicate. Bars, the standard error of the mean of each series.