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Figure 4 | Retrovirology

Figure 4

From: A suboptimal 5' splice site downstream of HIV-1 splice site A1 is required for unspliced viral mRNA accumulation and efficient virus replication

Figure 4

Effects of non-coding exon inclusion on viral mRNA stability. (A) RNase-protection mapping of HIV-1 spliced mRNAs using the DPHV riboprobe after transient transfection of the indicated plasmids and treatment with actinomycin D for the indicated times. Quantitation of the changes in accumulation of the spliced (B) viral mRNA species after the addition of actinomycin D (normalized to cellular β-actin) is shown relative to the onset of the experiment. The co-transfected pCMVβgal110 control was used to measure LacZ turnover, and the data shown represents LacZ mRNA levels within NL4-3 co-transfected cells. The data shown represent the average of three independent experiments.

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Retrovirology

ISSN: 1742-4690

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