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Figure 2 | Retrovirology

Figure 2

From: A peptide-loaded dendritic cell based cytotoxic T-lymphocyte (CTL) vaccination strategy using peptides that span SIV Tat, Rev, and Env overlapping reading frames

Figure 2

IFN-γ T-cell responses against the overlapping epitopes of Tat, Rev, and Env. Using an ELISpot assay, we measured IFN-γ T-cell responses against the peptides used in the vaccination protocol. The vaccinated animals (Panel A), AT56, AT57, AV89, and BA20, each developed strong to moderate responses against every peptide pool tested at a least one time point, except AT56 against Rev pool B. The control animals (Panel B), H405 and T687, did not demonstrate any significant activity throughout the study. The activity levels against the peptide pools, Tat A, Tat B, Rev A, Rev B, Env A, and Env B, are shown for each animal in spot forming cells (SFC) per 106 PBMC. Data are shown from pre-immunization and post-immunization assays. The average numbers of SFC per PBMC and the standard deviations (error bars) were determined from duplicate wells. Responses greater than 50 SFC/106 PBMC were considered positive.

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Retrovirology

ISSN: 1742-4690

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