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  • Oral presentation
  • Open Access

Preclinical Evaluation of Anti-HIV-1 Polybiguanide Vaginal Microbicides in a Murine Model of Toxicity and Inflammation

  • 1,
  • 2,
  • 2,
  • 1 and
  • 1
Retrovirology20052(Suppl 1):S98

https://doi.org/10.1186/1742-4690-2-S1-S98

Published: 8 December 2005

Keywords

  • Inflammatory Response
  • Temporal Difference
  • Safety Profile
  • Murine Model
  • Specific Marker

The use of vaginal microbicides has gained support as a strategy for the protection of women against HIV-1 and other sexually transmitted disease (STD) pathogens. The preclinical Swiss Webster murine model has been developed to specifically measure cervicovaginal tissue integrity and inflammation following application of candidate vaginal microbicides, when potential exposure to an STD pathogen may occur. This model demonstrates both mechanistic and temporal differences in inflammatory responses following microbicide exposure. Currently, specific markers of inflammation, including pro-inflammatory cytokines, are being evaluated in the cervicovaginal mucosa. Safety profiles of polybiguanides (PBGs), which demonstrated significant in vitro efficacy against HIV-1, are being assessed in vivo. Intravaginal application of PEHMB (1%) resulted in little or no cervicovaginal toxicity after short- or long-term exposure. Collectively, these studies support the Swiss Webster model as a valuable tool for the preclinical assessment of toxicity and inflammation associated with exposure to candidate topical microbicides. Furthermore, these results strongly support further development of polybiguanide derivatives as vaginal microbicidal agents.

Notes

Authors’ Affiliations

(1)
Department of Microbiology and Immunology, and Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, USA
(2)
Novaflux Biosciences, Inc., Princeton, USA

Copyright

© The Author(s) 2005

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