- Oral presentation
- Open Access
A Novel Post-transcriptional Block in Gene Expression Contributes to HIV-1 Latency In Vivo
- Published: 8 December 2005
Keywords
- Infected Cell
- Feedback Loop
- Nuclear Localization
- Proteomic Analysis
- Positive Feedback Loop
HIV-1 latency represents a major barrier to eradication. We describe a novel post-transcriptional block in HIV-1 gene expression in latently infected cells. Multiply spliced HIV-1 RNAs encoding the critical positive regulators Tat and Rev exhibited strict nuclear localization in latently infected primary resting CD4+ T cells. Proteomic analysis identified polypyrimidine tract binding protein (PTB) as a HIV-1 RNA binding protein which allows cytoplasmic accumulation of HIV-1 RNAs and subsequent release of replication-competent virus by latently infected cells. Thus a post-transcriptional block in resting cells interrupts a positive feedback loop and contributes to latency. This works suggests novel approaches for reversing latency.
Notes
Authors’ Affiliations
(1)
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, US
(2)
Howard Hughes Medical Institute, Baltimore, MD, 21205, US
Copyright
© The Author(s) 2005
