- Oral presentation
- Open Access
A Novel Post-transcriptional Block in Gene Expression Contributes to HIV-1 Latency In Vivo
Retrovirology volume 2, Article number: S74 (2005)
HIV-1 latency represents a major barrier to eradication. We describe a novel post-transcriptional block in HIV-1 gene expression in latently infected cells. Multiply spliced HIV-1 RNAs encoding the critical positive regulators Tat and Rev exhibited strict nuclear localization in latently infected primary resting CD4+ T cells. Proteomic analysis identified polypyrimidine tract binding protein (PTB) as a HIV-1 RNA binding protein which allows cytoplasmic accumulation of HIV-1 RNAs and subsequent release of replication-competent virus by latently infected cells. Thus a post-transcriptional block in resting cells interrupts a positive feedback loop and contributes to latency. This works suggests novel approaches for reversing latency.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Lassen, K.G., Ramyar, K.X., Bailey, J.R. et al. A Novel Post-transcriptional Block in Gene Expression Contributes to HIV-1 Latency In Vivo. Retrovirology 2 (Suppl 1), S74 (2005). https://doi.org/10.1186/1742-4690-2-S1-S74
- Infected Cell
- Feedback Loop
- Nuclear Localization
- Proteomic Analysis
- Positive Feedback Loop