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Baculovirus-Derived HIV-1 Virus-Like Particles (VLP) Activate Dendritic Cells and Are Cross-Presented to Induce In Vitro T-Cell Response
Retrovirology volume 2, Article number: S70 (2005)
Aim
to evaluate the ability of the baculovirus-expressed HIV-VLPA to induce the maturation and activation of monocyte-derived dendritic cells (MDDCs).
Results
the VLP-activated MDDCs show an enhanced Th1 and Th2-specific cytokine production and the effects of VLPs on MDDCs are, to some extent, mediated through intra-cellular Toll-like receptors signaling. The VLP-loaded MDDCs are able to induce a primary and secondary response in autologous T cells, using an in vitro immunization assay. Moreover, the genomic transcriptional profile of VLP-activated DCs show, by gene microarray analysis, the upregulation of several genes involved in the immune response.
Conclusion
Our results show that baculovirus VLPs activate MDDCs and may "cross" over to the endogenous pathway to gain access to MHC class I, inducing CD8+ cytotoxic T cells activation. The intra-cellular Toll-like receptors appear to be involved in this process; additional signaling pathways induced by VLPs in the MDDCs are currently under evaluation. These data give an insight into the mechanisms of the cellular immunity induced in vivo by VLPs, which may be extremely useful to optimize and modulate the immune response.
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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Buonaguro, L., Tornesello, M., Aricò, E. et al. Baculovirus-Derived HIV-1 Virus-Like Particles (VLP) Activate Dendritic Cells and Are Cross-Presented to Induce In Vitro T-Cell Response. Retrovirology 2 (Suppl 1), S70 (2005). https://doi.org/10.1186/1742-4690-2-S1-S70
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DOI: https://doi.org/10.1186/1742-4690-2-S1-S70
Keywords
- Dendritic Cell
- Microarray Analysis
- Cytokine Production
- Transcriptional Profile
- Cellular Immunity