Skip to main content

Advertisement

HIV-1 Specific CD4 and CD8 T-cell Responses Associated With Low Viral Load in Treatment-Naïve HIV-1 Infected Individuals

Article metrics

  • 867 Accesses

In preparation for monitoring of vaccine-induced responses, we determined HIV-specific cell-mediated immune responses in 17 treatment naïve HIV-1 infected individuals with > 400 CD4+ T cells/ml for at least 5 years including 9 patients with low viral load (VL, < 5000 copies/ml) and 8 with high VL (> 5000 copies/ml). HIV-1 specific IFN-γ-production and cytolytic activity were higher in subjects with low VL. The differences between the two groups were statistically significant for CD4+ T-cell responses to Gag and Nef peptides, tested by a long-term (48 h) ICS assay and of border-line significance for the Gag-specific cytolytic responses measured by a flow-cytometry assay and a chromium release assay. We also found a significant inverse correlation between VL and IFN-γ-production by CD8+ T-cells in response to Gag as measured by ICS. The ELISpot IFN-γ response was not significantly different in patients with high and low VL. During a median follow-up period of 2.4 years, 6 of 8 subjects with high VL and 1 of 9 with low VL showed decreasing CD4+ T-cell counts, and ARV treatment was more frequently initiated in the former patient group (5 of 8 versus 1 of 9). The CD4 and CD8 T cell immune responses found to be associated with low VL and stable CD4 counts may be of importance for protection.

Author information

Correspondence to Gunnel Biberfeld.

Rights and permissions

Reprints and Permissions

About this article

Keywords

  • Cell Immune Response
  • Chromium Release
  • Chromium Release Assay
  • Cytolytic Response