- Poster presentation
- Open Access
Primary HIV-1 Infection Sets the Stage for Important B Lymphocyte Dysfunctions
Retrovirology volume 2, Article number: P95 (2005)
B lymphocytes of patients with chronic HIV-1 infection (CHI) show functional and phenotypic abnormalities. We investigated the effects of primary HIV-1 infection (PHI) on activation, differentiation and survival of B cells. The effects of antiretroviral therapies on B cell dysfunctions in PHI were also studied.
Design and Methods
B cells of 31 PHI patients (sampled at baseline, 1 month and 6 months post therapy), 26 CHI patients, and 12 healthy donors were studied for surface expression of Fas, LAIR-1, CD70, intracellular expression of Bcl-2, and spontaneous apoptosis. Four-colour FACS (IgD+IgM+CD19+CD27), and short-term PBMC cultures to analyse induction of CD25 on B cells were performed in 5 PHI patients.
In PHI, naïve and memory B lymphocytes were highly activated, manifested by hypergammaglobulinemia, altered expression of Fas and LAIR-1, and increased spontaneous apoptosis. Antiretroviral treatment improved the activation/differentiation status of B cells, reduced apoptosis to levels comparable to healthy individuals and restored the ability of B cells to respond to T-cell dependent activation. B cells of PHI patients on HAART recovered better compared to patients on RTI only. Data obtained on 5 PHI patients at baseline showed decreased IgM+ memory B cells and lower induction of CD25 expression on B cells upon T cell activation. These parameters were normalized after 6 months of antiretroviral treatment.
B cell dysfunctions in HIV-1 infection appear during primary infection and initiation of antiretroviral therapy early during infection may help preserve the B cell compartment.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Titanji, K., Chiodi, F., Bellocco, R. et al. Primary HIV-1 Infection Sets the Stage for Important B Lymphocyte Dysfunctions. Retrovirology 2 (Suppl 1), P95 (2005). https://doi.org/10.1186/1742-4690-2-S1-P95
- CD25 Expression
- Healthy Donor
- Primary Infection
- Cell Compartment
- Cell Dysfunction