Skip to content


  • Poster presentation
  • Open Access

Phage Display Selection of HIV Specific Conserved Mimotopes With IgG from Long-term Non-progressors

  • 1,
  • 1,
  • 1,
  • 2,
  • 2,
  • 3 and
  • 1Email author
Retrovirology20052 (Suppl 1) :P29

  • Published:


  • Peptide
  • Immune Response
  • Infectious Disease
  • Cancer Research
  • Neutralize Antibody


The aim of this study is to identify conserved epitopes of HIV-1 neutralizing antibodies in polyclonal plasma from LTNP to finally derive vaccine candidates.

Materials and methods

The presence of neutralizing antibodies in 9 LTNP sera was proved by in vitro neutralization assays. Phage displayed peptide libraries were screened with LTNP IgG. HIV-specific mimotopes were analyzed for homology to the gp120 structure by a software (3DEX) especially developed for this purpose. Mice were immunized with interesting phages and their sera were analyzed for neutralizing activities against HIV-1.


After biopannings, between 19% and 75% HIV-specific phage clones were identified by ELISA. Mimotope sequences were identified and could be aligned by 3DEX to linear or conformational epitopes on gp120. A peptide specific immune response was detected in sera of immunized mice. The first mice sera analyzed showed neutralizing activities against HIV-1.


Mimotopes could be selected from LTNP sera that represent conformational epitopes on gp120. Those ones inducing neutralizing antibodies upon immunization potentially are suited to derive vaccine candidates.


Authors’ Affiliations

Georg-Speyer-Haus, Frankfurt, Germany
Instituto de Salud Carlos III, Madrid, Spain
Virology, University of Heidelberg, Heidelberg, Germany


© The Author(s) 2005