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  • Poster presentation
  • Open Access

Phage Display Selection of HIV Specific Conserved Mimotopes With IgG from Long-term Non-progressors

  • 1,
  • 1,
  • 1,
  • 2,
  • 2,
  • 3 and
  • 1Email author
Retrovirology20052 (Suppl 1) :P29

https://doi.org/10.1186/1742-4690-2-S1-P29

  • Published:

Keywords

  • Peptide
  • Immune Response
  • Infectious Disease
  • Cancer Research
  • Neutralize Antibody

Background

The aim of this study is to identify conserved epitopes of HIV-1 neutralizing antibodies in polyclonal plasma from LTNP to finally derive vaccine candidates.

Materials and methods

The presence of neutralizing antibodies in 9 LTNP sera was proved by in vitro neutralization assays. Phage displayed peptide libraries were screened with LTNP IgG. HIV-specific mimotopes were analyzed for homology to the gp120 structure by a software (3DEX) especially developed for this purpose. Mice were immunized with interesting phages and their sera were analyzed for neutralizing activities against HIV-1.

Results

After biopannings, between 19% and 75% HIV-specific phage clones were identified by ELISA. Mimotope sequences were identified and could be aligned by 3DEX to linear or conformational epitopes on gp120. A peptide specific immune response was detected in sera of immunized mice. The first mice sera analyzed showed neutralizing activities against HIV-1.

Conclusion

Mimotopes could be selected from LTNP sera that represent conformational epitopes on gp120. Those ones inducing neutralizing antibodies upon immunization potentially are suited to derive vaccine candidates.

Notes

Authors’ Affiliations

(1)
Georg-Speyer-Haus, Frankfurt, Germany
(2)
Instituto de Salud Carlos III, Madrid, Spain
(3)
Virology, University of Heidelberg, Heidelberg, Germany

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