Characterization of Proviral HIV Latency in Different T Cell Subsets of Patients Undergoing HAART
Retrovirology volume 2, Article number: P151 (2005)
In an HIV infected person, each body compartment harbors a distinct resident HIV. There is an increasing awareness that each T cell subset harbors a genetically distinct lineage of the virus.
Materials and methods
Peripheral blood was obtained from 5 HIV patients receiving HAART for 2–12 years. Three patients had 400<HIV RNA copies/ml with between 529 and 1,588 CD4 T cells/μl. Other two had 915 and 453,000 RNA copies/ml with 235 and 266 CD4 T cells/μl. Each T cell subset was sorted by FACSAria, washed and DNA was isolated. Proviral HIV env C2-V3 genes were PCR amplified, cloned and sequenced, and were phylogenetically analyzed by using MEGA (v.2.1).
In each individual, different T cell subsets harbored genetically distinct lines of HIV. In most patients, CD45RO (memory) subset of CD4 T cells were positive for HIV proviral DNA. Only one patient was positive for proviral HIV in naïve CD4 T cells. Both naïve CD4 and CD8 T cells showed highly divergent proviral HIV sequences.
In patients receiving a long-term HAART, proviral HIV DNA in each T cell subset represented a distinct lineage of the virus. Even in patients with less than detectable levels of HIV in the plasma, proviral DNA showed the evidence of drug resistance to antiretrovirals.
About this article
Cite this article
Chaudhary, S., Lopez, P., Rodriguez, N. et al. Characterization of Proviral HIV Latency in Different T Cell Subsets of Patients Undergoing HAART. Retrovirology 2 (Suppl 1), P151 (2005). https://doi.org/10.1186/1742-4690-2-S1-P151