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  • Poster presentation
  • Open Access

Circulating Human Vγ2/Vδ2 T Cells Express Cytoplasmic RANTES

  • 1,
  • 1,
  • 2,
  • 1 and
  • 2
Retrovirology20052 (Suppl 1) :P145

https://doi.org/10.1186/1742-4690-2-S1-P145

  • Published:

Keywords

  • Effector Memory
  • Central Memory
  • Cytoplasmic Granule
  • Memory Phenotype
  • Memory Pool

A likely process of chronic positive selection produces the highly biased peripheral blood γδ T-cell repertoire of adult human beings. The major blood subset expresses the Vγ2Vδ2 T-cell receptor and responds to phosphoantigen stimulation in the absence of MHC restriction. Chronic expansion of γδ T-cell pool is expected to produce a population of cells with the effector/memory phenotype. A CC chemokine RANTES is produced late after TCR stimulation of αβ T-cells, accumulates into the cytoplasm and represents a marker for non-naïve T-cells. We demonstrate here that the vast majority of peripheral human T-cells contain RANTES in the cytoplasmic granules. In vitro expansion after non-peptidic phosphoantigen stimulation mimics the normal γδ T cell response to pathogens, and produces polyclonal Vγ2/Vδ2 cell population uniformly positive for cytoplasmic RANTES. These cells readily release RANTES from cytoplasmic depots into the culture medium after TCR stimulation. The presence of stored RANTES suggests a memory phenotype and may mediate effector functions of circulating Vγ2/Vδ2 cells. Phosphoantigen-responsive Vγ2/Vδ2 T cells represent 1 in 40 of circulating CD3+ lymphocytes; this is the dominant central memory population in primate peripheral blood that can evolve directly into an effector memory pool.

Notes

Authors’ Affiliations

(1)
University of Maryland Biotechnology Institute, Institute of Human Virology, USA
(2)
Medical Biotechnology Center, University of Maryland Biotechnology Institute, USA

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