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  • Open Access

Replication of Significant Relationship Between MIP-1β Production Following p24 Stimulation and Type C Coping

  • 1, 2Email author,
  • 2,
  • 2,
  • 2 and
  • 3
Retrovirology20052(Suppl 1):P114

https://doi.org/10.1186/1742-4690-2-S1-P114

Published: 8 December 2005

Keywords

  • Infectious Disease
  • Cancer Research
  • Longitudinal Study
  • Significant Relationship
  • Similarity Rate

Background

We have shown that the Type C style of coping with stress (diminished ability to recognize and express stress/ distress/emotions) is related to HIV progression. We recently found in a study of 50 HIV+ patients in Baltimore, that stronger Type C coping is associated with decreased production of beta-chemokines that bind to the HIV co-receptor CCR5. Under the aegis of NIH, we have initiated a longitudinal study with a final N = 200 to evaluate the core hypothesis that lower production of the beta-chemokines MIP-1 α/β mediates the relationship between Type C coping and HIV progression.

Methods

Type C coping was assessed using Temoshok's Vignette Similarity Rating Method. Measurement of antigen-induced chemokine production from subjects' blood followed methods described in Garzino-Demo et a. PNAS 1999. Cells were incubated with media alone (control), p24 antigen, PHA, or candida. Supernatants were collected on day 3 and 6 for beta- chemokine measurements. Assays for MIP-1α/β were performed by commercial ELISA for the first 47 subjects.

Results

Subjects who scored high on Type C coping (3,4,5) had a significantly lower mean stimulation index for MIP-1β by p24, compared to subjects low on Type C coping (2.7 vs. 5.0).

Conclusion

This replication in a separate sample strengthens our hypothesis about mechanisms mediating HIV progression.

Notes

Authors’ Affiliations

(1)
Dept Medicine, Univ Maryland School Med, Baltimore, USA
(2)
Institute of Human Virology, Univ Maryland Biotechnol Inst, USA
(3)
Public Research Institute, San Francisco State Univ, USA

Copyright

© The Author(s) 2005

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