- Poster presentation
- Open Access
Design of RANTES-derived Peptides With Enhanced HIV-inhibitory Activity and Derivation of Resistant HIV-1 Strains
Retrovirology volume 2, Article number: P113 (2005)
We previously identified the major structural determinants of CCR5 binding and HIV blockade in RANTES, describing linear RANTES-derived peptides with biological activity in the low micromolar range (Nardese et al., 2001). To deepen our understanding of RANTES structure-function relations and obtain more potent antiviral peptido-mimetics, we have extensively mutagenized the prototypic peptide, R11-29. This presents two clusters of hydrophobic residues at its termini, (corresponding to RANTES N-loop and b1-strand) connected by a positively-charged linker. Single or multiple alanine substitutions within the N- or C-terminal hydrophobic clusters resulted in a dramatic loss of antiviral activity, whereas deletion of selected residues within the hydrophilic linker had no major functional consequences. Based on RANTES 3D structure, we designed a series of modified peptides, resulting in a progressive increase in specific antiviral activity. These peptides also displayed anti-inflammatory properties blocking RANTES-elicited lymphocyte chemotaxis. Through serial passages in culture in the presence of increasing concentrations of the most effective antiviral peptides, we have derived variants of the R5 HIV-1 isolate BaL resistant to the peptide inhibitory activity. Complete sequencing of the envelope genes from such variants is currently underway. Our results provide new insights into the structure of the receptor-binding region of RANTES and identify new antiviral peptides that may be instrumental in the development of effective HIV-1 entry inhibitors.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Vangelista, L., Longhi, R., Tolazzi, M. et al. Design of RANTES-derived Peptides With Enhanced HIV-inhibitory Activity and Derivation of Resistant HIV-1 Strains. Retrovirology 2 (Suppl 1), P113 (2005). https://doi.org/10.1186/1742-4690-2-S1-P113
- Antiviral Activity
- Envelope Gene
- Hydrophobic Cluster
- Entry Inhibitor
- Alanine Substitution