Targeted infection of HIV-1 Env expressing cells by HIV(CD4/CXCR4) vectors reveals a potential new rationale for HIV-1 mediated down-modulation of CD4

Table 1 Neutralization of vector infectivity by antibodies to CD4 and VSV G^a

Antibody:	None^c		anti-CD4^c		anti-VSV G^c
Particle^b	Env⁺	Env^-	Env⁺	Env^-	Env⁺	Env^-
HIV-1-Neo(w/o CD4)	3	4	4	0	3	4
HIV-1-Neo(CD4)	403	5	6	3	401	3
HIV-1-Neo(CD4/gpi)	363	2	7	3	391	4
HIV-1-Neo(G)	591	517	446	550	4	6

^a10⁵ Env⁺ TF228 or 10⁵ Env^- BJAB cells were infected and neomycin-resistant cell colonies were selected in soft agar and counted after two weeks. The average number of selected colonies is presented from at least two titrations.
^b Vector particles were collected from human 293 cell supernatants after single DNA transfection with HIV-1-Neo or after cotransfection of HIV-1-Neo DNAs with DNAs encoding either CD4, CD4/gpi or the glycoprotein G of vesicular stomatitis virus, respectively.
^c1 ng p24 of each vector type was preincubated for 30 min with or without 2 mg/ml antibody directed either against the CD4 ectodomain or the vesicular stomatitis virus glycoprotein G prior to infections.

ISSN: 1742-4690