Real time quantitative PCR (qPCR) analysis of hTERT expression in HTLV-1-infected and uninfected T cells. A) ATL cell lines (HTLV-1 infected but negative for Tax expression); B) In vitro transformed cell lines (HTLV-1 infected and positive for Tax expression); C) Jurkat T-cell clones positive for Tax expression (E12, C11 and C50); D) Uninfected primary CD4 T lymphocytes either activated or not (resting); T-cell line (DCH-4) immortalized with a lentivirus vector encoding a Tax-YFP fusion protein; Infected T lymphocytes isolated from patient suffering from TSP/HAM. Cytoplasmic RNAs were isolated, reverse transcribed and cDNA were analyzed by qPCR using primers for hTERT and PBGD. Results are expressed as indicated in legend of table 1. Standard deviations are from at least three determinations performed in duplicate.