Figure 3

Time and dose effects of Tat-P on recombinant lentiviral vector production. Panel A. To investigate the Tat-P driven inhibition of lentiviral vector generation over time, supernatants from the viral particle producing 293T cell cultures, in the presence of 200 μM of Tat-P, Con-P1, Con-P2, and PBS, were collected at 6 hour, 12 hour, and 24 hour time points. The supernatants containing the viral particles were added to freshly cultured 293T cell and these supernatants were evaluated for virus titers. Panel B. To assess dose-dependency of the Tat-P inhibition activity, supernatants from the viral particle producing 293T cell cultures, in the presence of 200 μM, 100 μM, and 50 μM of Tat-P and Con-P1, were collected at the 6-hour time point. Viral titers were determined and the inhibition effects were calculated.