Figure 4

In vivo transduction of cells using pREV-HW3 vector in the cerebellum. 4 days post injection, histochemical staining of PLAP in brain sections shows labeled cells in the region occupied by neural precursors in the external granular layer of the cerebellum, observed as discrete cells (A and B) or patches of staining (C). Panel B shows the region bordered by the black rectangle in A at higher magnification. 15 dpi histochemistry revealed transduced cells in patches and scattered about the parenchyma of the cerebellum (D, E and F). The majority of cells were found in the internal granular layer (E and F) but cells were also found in and astride bands of cerebellar white matter which is indicated by a black arrow (D). Discrete cells often exhibit a clearly neuronal morphology (F), the thin arrow indicates a cell with the morphology of a granular neuron, the arrow head indicates a cell with the morphological characteristics of a cerebellar golgi neuron. PCL, purkinje cell layer. Scale bars are 200 μm (A), 100 μm (B, C, D), and 40 μm (E, F)