Figure 5

WD5 domain of LIS1 interact with HIV-Tat. LIS1, WD5 domain of LIS1 (WD5) and N-terminal portion of LIS1 (LIS1 NT) were translated in reticulocyte lysate containing [³⁵S] methionine as described in the Experimental procedures section. Lysates were precipitated with GST-fused Tat 1–72, Tat 1–48, Tat 37–72 or GST alone, immobilized on glutathione-agarose beads, and resolved on 12% SDS-PAGE. Lanes 1, 6 and 11 – Input; Lanes 2, 7 and 12 – precipitation of LIS1, WD5 or LIS1 NT with Tat 1–72; Lanes 3 and 8 – precipitation of LIS1 and WD5 with Tat 1–48; Lanes 4 and 9 – precipitation of LIS1 and WD5 with Tat 37–72; Lanes 5, 10 and 13 – precipitation of LIS1, WD5 or LIS1 NT with GST alone. The figure is an autoradiogram.