Figure 3

Deletion of two regions in CDK4 interferes with Tax-binding in vivo . A) Tax and CDK4 mutants were coexpressed in transfected 293T cells. The complexes were immunoprecipitated by monoclonal Tax antibodies and protein A sepharose. To detect Tax-bound CDK4 mutants, complexes and lysate controls were subjected to gel-electrophoresis and Western blotting. One representative experiment is shown. B) Luminescence emitted by specific bands of two independent experiments was quantitative evaluated and the mean relative Tax binding was calculated.