Figure 4

Transgenic macrophages display normal cell surface markers: GFP-alone and XHR transduced CD34+ derived macrophages were labeled with antibodies specific for (A) CD14, (B) CD4, and (C) HLA-DR and analyzed by FACS. Control, nontransduced macrophages are shown superimposed as unshaded areas. (D) B7.1 upregulation of transgenic macrophages stimulated with LPS. Twenty-four hours post-stimulation, macrophages were labeled with a PE-CY5 conjugated anti-B7.1 antibody and analyzed by FACS. B7.1 upregulation data are representative of triplicate experiments.