Figure 2
From: Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera
A. Western blot analysis of HeLa cells untransfected (lanes 1, 4), or transfected with an expression vector for HIV-1 Env and Tat plus either pNH-YFPgpi (lanes 2, 5) or pYFPgpi as control (lanes 3, 6). Left side, cell lysates analyzed with rabbit anti-gp120 antiserum (upper panel), or anti-actin as loading control (lower panel). Right side, cell surface proteins labeled with NHS-biotin, precipitated with avidin-agarose, and analyzed with rabbit anti-gp120 antiserum (upper panel), or anti-integrin α5 as loading control (lower panel). One of two independent experiments with similar results is shown. B. Cell fusion assay. Indicator HeLa-TZM cells (CD4+, CXCR4+, CCR5+, containing an HIV LTR-luciferase reporter) were cultured overnight with HEK293 cells untransfected (left bar) or transfected with an expression vector for pNL4-3 strain HIV-1 Env and Tat, plus either pNH-YFPgpi (middle bar) or pYFPgpi (right bar) and analyzed for luciferase activity. RLU, relative light units. One of four independent experiments with similar results is shown.