Figure 4

Tat induces cytochrome c release from isolated mitochondria. After 2 hour treatment with 10 μM (P10) of peptide 73–99 or 0.2 μM (T0.2), 2 μM (T2) and 10 μM (T10) of Tat Eli or without peptide (T-), samples were centrifuged and supernatants and pellets were separated. (A). Western blots of the supernatant with antibodies against cytochrome c (Cyt C) and with antibodies against VDAC. (B) Western blots of Mitochondria Pellets. Data are representative of four independent experiments.