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Figure 2 | Retrovirology

Figure 2

From: Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription

Figure 2

Binding of Tat to TAR RNA and CDK9/cyclin T1. Precipitation of biotin TAR RNA with purified Tat and with CDK9/cyclin T1. Lane 1, control denatured TAR RNA. Lane, control without Tat. Lane 3, untreated CDK9. cyclin T1. Lane 4, CDK9/cyclin T1 treated with PP2A (panel A) or with PP1 (panel B). Precipitated proteins and TAR RNA were recovered in SDS-loading buffer, resolved 12% SDS-PAGE and immunoblotted with indicated antibodies. Position of TAR RNA was determined by Ponceau-S staining.

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