Figure 2
From: HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain
The intracellular steady state localization of the wild type Rev and mutants is shown in the panels to the left (panels a-e). The panels to the rights show the nuclear accumulation of the wild type and mutant proteins after treatment with 5 nM LMB for 6 hours (panels f-j). The anti-Rev Mab 8E7 combined with FITC labeled anti-mouse IgG2a (Southern Biotech) was used for detection of Rev and the M4 mutants.