Skip to main content

Proteomic profiles among asymptomatic HTLV-1 carriers and HAM/TSP patients in Peru

High proviral loads have been described in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients in comparison to asymptomatic HTLV-1 carriers (AC). However, biomarkers related to HAM/TSP progression have not been identified. We analyzed differential proteome changes by two-dimensional gel electrophoresis (2D-electrophoresis) to identify spots of proteins in plasma samples of three groups of patients: five AC, nine HAM/TSP patients: five patients with EDSS scores of 1.0-5.0 (=mild HAM/TSP) and 4 patients with EDSS scores of 5.5-9.0 (=severe HAM/TSP). Proteins were extracted from pooled plasma samples from each group of patients. Protein separations were performed by electrofocusing with 11 cm strips at 3-10 pH range, electrophoresis with 12% poliacrilamide gel electrophoresis and silver staining. Analyses were performed in Progenesis Same Spot software; ANOVA was used to compare the profiles of the groups. Six spots of proteins were differentially expressed among these groups: five proteins increased their expression according to HAM/TSP disease progression (highest fold change= 6.1, p=1.671 x10-4; lowest fold change= 1.5, p= 0.014) while one protein was highly expressed in AC and decreased according to HAM/TSP progression (fold change= 5.8, p= 0.002). Further studies to confirm the expression of these proteins in a larger set of samples are still in progress, the identification of these proteins will be performed by mass spectrometry. These results might be promissory to identify biomarkers for HAM/TSP progression.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Michael Talledo.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Temoche, M., Rosado, J., Alvarez, C. et al. Proteomic profiles among asymptomatic HTLV-1 carriers and HAM/TSP patients in Peru. Retrovirology 11, P127 (2014). https://doi.org/10.1186/1742-4690-11-S1-P127

Download citation

Keywords

  • Plasma Sample
  • Fold Change
  • Silver Staining
  • Proteomic Profile
  • Protein Separation