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  • Open Access

Proteomic profiles among asymptomatic HTLV-1 carriers and HAM/TSP patients in Peru

  • 1, 2,
  • 1, 2,
  • 2, 3,
  • 2, 4,
  • 2, 5, 6 and
  • 1, 2Email author
Retrovirology201411 (Suppl 1) :P127

https://doi.org/10.1186/1742-4690-11-S1-P127

  • Published:

Keywords

  • Plasma Sample
  • Fold Change
  • Silver Staining
  • Proteomic Profile
  • Protein Separation

High proviral loads have been described in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients in comparison to asymptomatic HTLV-1 carriers (AC). However, biomarkers related to HAM/TSP progression have not been identified. We analyzed differential proteome changes by two-dimensional gel electrophoresis (2D-electrophoresis) to identify spots of proteins in plasma samples of three groups of patients: five AC, nine HAM/TSP patients: five patients with EDSS scores of 1.0-5.0 (=mild HAM/TSP) and 4 patients with EDSS scores of 5.5-9.0 (=severe HAM/TSP). Proteins were extracted from pooled plasma samples from each group of patients. Protein separations were performed by electrofocusing with 11 cm strips at 3-10 pH range, electrophoresis with 12% poliacrilamide gel electrophoresis and silver staining. Analyses were performed in Progenesis Same Spot software; ANOVA was used to compare the profiles of the groups. Six spots of proteins were differentially expressed among these groups: five proteins increased their expression according to HAM/TSP disease progression (highest fold change= 6.1, p=1.671 x10-4; lowest fold change= 1.5, p= 0.014) while one protein was highly expressed in AC and decreased according to HAM/TSP progression (fold change= 5.8, p= 0.002). Further studies to confirm the expression of these proteins in a larger set of samples are still in progress, the identification of these proteins will be performed by mass spectrometry. These results might be promissory to identify biomarkers for HAM/TSP progression.

Authors’ Affiliations

(1)
Laboratorio de Epidemiología Molecular, Universidad Peruana Cayetano Heredia, Lima, Peru
(2)
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru
(3)
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium
(4)
Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru
(5)
Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Peru
(6)
Hospital Nacional Cayetano Heredia, Lima, Peru

Copyright

© Temoche et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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