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Modulation of spontaneous proliferation of T-lymphocytes from HTLV-1- infected individuals by quinoline compounds

Spontaneous proliferation, a hallmark of Human-T Lymphocyte Virus Type 1 (HTLV-1) infection, is particularly higher in HAM/TSP patients compared to asymptomatic carriers. However the role of the proliferation in the pathogenesis of HAM/TSP is still unknown. The identification of drugs that modulate the spontaneous proliferation may be important for the treatment of HAM/TSP disease. We have evaluated the effect of three different quinoline compounds (A, B and C) on the modulation of spontaneous proliferation of peripheral blood mononuclear cells from HTLV-1 patients with HAM/TSP. The cells from 8 HAM/TSP patients were cultivated in the presence of serial concentrations of quinolone compounds. Cell proliferation was assessed by 3[H]thymidine incorporation. Cell viability was measured by optical density in the presence of MTT. The ultrastructure analysis was done using a transmission electron microscope. Quinoline compounds were not toxic at the concentrations evaluated. The IC50 was 18.5 µM for compound A, 30.5 µM for compound B and 3.4 µM for compound C. The three compounds inhibited more than 90% of spontaneous proliferation. Cultured cells in the presence of quinolone compounds showed vacuoles presented with myelin-like membranes, probably autophagic vacuole-like compartments, observed by electronic microscopy. In conclusion, the quinoline compounds showed no toxicity and were able to inhibit the spontaneous proliferation of T cells from HTLV-1-infected individuals. New assessments are being applied to understand how the quinoline compounds act on cells by decreasing cell proliferation.

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Correspondence to Maria FR Grassi.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated.

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Pinto, L.A., Vannier-Santos, M.A., Fournet, A. et al. Modulation of spontaneous proliferation of T-lymphocytes from HTLV-1- infected individuals by quinoline compounds. Retrovirology 11 (Suppl 1), P121 (2014).

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