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HTLV-1 infection in bone marrow mesenchymal stem cells isolated from HTLV-1 individuals

The characterization of cell types that are susceptible to HTLV-1 infection is essential to understand of the biology of virus infection and the pathophysiology of HTLV-related diseases. In the present study, we investigated bone marrow (BM) cells from asymptomatic carriers (HAC) and symptomatic HTLV-1 individuals. Initially, we observed an infiltrated of CD4+ T-cell lymphocytes in BM from HTLV-1 individuals when compared to healthy controls (p≤0.02). The proviral DNA of BM HTLV-1 CD4+ T cells revealed the presence of integrated provirus. The number of fibroblast progenitor cells, referred as colony-forming units-fibroblasts (CFU-F), were lower in HTLV-1 infected individuals (CFU-F per 5 x 105 cells in HAM/TSP (2.1±1.6), HAC (7.5±2.1) compared to healthy controls (10.4±1.1). HTLV-1 BM mesenchymal stem cells (MSC) isolated showed surface expression of CD105, CD73, and CD90, absence of hematopoietic markers such as CD45, CD34, and CD14; and in vitro differentiation to adipogenic and osteogenic cells. Proviral HTLV-1 DNA was detected in MSC from all HTLV-1 patients and the levels ranged from 2.5 to 25.7 copies per 105 cells. HTLV-1 MSC was also positive to HTLV-1 p19 protein as determined by confocal microscopy. The confirmation of active viral replication was performed in the concentrated supernatant obtained from MSC by real-time PCR of the pol and gag HTLV-1 gene. In conclusion, we suggest that HTLV-1 could infect and replicate in human MSC possibly by the contact with infected CD4+ T-cells.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated.

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Rodrigues, E.S., do Carmo Favarin, M., de Macedo, M.D. et al. HTLV-1 infection in bone marrow mesenchymal stem cells isolated from HTLV-1 individuals. Retrovirology 11 (Suppl 1), P106 (2014).

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