Volume 11 Supplement 1

16th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access

Characterization of glycans of CD4+T cells in HAM/TSP

  • Daisuke Kodama1Email author,
  • Kimiko Izumo1,
  • Ryuji Kubota1,
  • Toshio Matsuzaki2,
  • Hiroshi Takashima3 and
  • Shuji Izumo1
Retrovirology201411(Suppl 1):O69

https://doi.org/10.1186/1742-4690-11-S1-O69

Published: 7 January 2014

The outermost structure of cells is galectin-glycan lattice, that is shown to have many roles in cell-cell interactions. Recently, biofilm-like extracellular viral assemblies were shown to mediate HTLV-1 cell-to-cell transmission. Here, we tried to characterize the glycans on the CD4+T cells in HAM. CD4+T cells from four respective cases of HAM, AC(asymptomatic carriers), and NC(negative control) were subjected to lectin array analysis. Similarly, glycans liberated from membrane proteins of CD4+T cells from six respective cases of HAM and NC analyzed by MALDI-TOF MS. We found that UDA(Urtica dioica agglutinin) and STL(Solanum tuberosum lectin (Potato)) lectins, that recognize N-glycan polylactosamine which consist of repeats of the disaccharide betaGal(1-4)betaGlcNAc(1-3), were significantly high in HAM in lectin array analysis. Interestingly, UDA was reported to inhibit cell-to-cell infection in vitro. On the other hand, MALDI-TOF MS analysis found several O-glycans in HAM. Candidates verified in GlycoSuite database were mainly O-glycans attach to MUC1 and Leukosialin(CD43) as carrier proteins. These proteins were reported to play roles in ATL and cell-to-cell infection as well. These glycans and carrier proteins may be therapeutic target of HAM.

Authors’ Affiliations

(1)
Molecular Pathology, Center for Chronic Viral Diseases, Kagoshima University Graduate School of Medical and Dental Sciences
(2)
Ohkatsu Hospital, Medical Corporation Sanshukai
(3)
Department of Neurology, Kagoshima University Graduate School of Medical and Dental Sciences

Copyright

© Kodama et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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