Volume 11 Supplement 1

16th Interntional Conference on Human Retroviruses: HTLV and Related Viruses

Open Access

The phase-I study of a therapeutic vaccine to ATL patients with autologous dendritic cells pulsed with peptides corresponding to Tax-specific CTL epitopes

  • Youko Suehiro1Email author,
  • Atsuhiko Hasegawa2,
  • Tadafumi Iino3,
  • Amane Sasada2,
  • Nobukazu Watanabe4,
  • Ilseung Choi1,
  • Tetsuya Fukuda5,
  • Shigeo Takaishi6,
  • Ryuji Tanosaki7,
  • Atae Utsunomiya8,
  • Osamu Miura5,
  • Masao Matsuoka9,
  • Takanori Teshima10,
  • Koichi Akashi3,
  • Jun Okamura11,
  • Mari Kannagi2 and
  • Naokuni Uike1
Retrovirology201411(Suppl 1):O2

https://doi.org/10.1186/1742-4690-11-S1-O2

Published: 7 January 2014

In the last decade, various kinds of clinical trials of hematopoietic stem cell transplantation (HSCT) have been performed for ATL patients, and showed therapeutic effects with long-term remission in a part of recipients. Our previous finding of activation of CD8+ Tax-specific cytotoxic T-lymphocytes (CTL) in post-HSCT ATL patients indicated the presence of Tax expression in vivo and potential contributions of CTL to GVL effects. These findings let us attempt developing an anti-ATL therapeutic vaccine consisting of autologous dendritic cells (DC) pulsed with Tax peptides corresponding to the major epitopes of Tax-specific CTL previously identified from HLA-A2, A24 or A11-possessing post-HSCT ATL patients. In preliminary experiments, the DC induced with a conventional method showed matured phenotype and produced IL-12 in 2 of 3 ATL patients tested. Under the official approval by the institutional ethical committees, we conducted the phase-I clinical study of anti-ATL immunotherapy for ATL patients at stable conditions after other therapy. The peptide-pulsed DC was subcutaneously injected for three times with 2 weeks intervals. The first patient showed a significant reduction in the proviral loads in 1 week after administration of DC, which gradually increase during the treatment, then decreased again at 8 weeks after the initiation of the therapy. Reduction in the size of surface lymph nodes by CT confirmed the therapeutic effects. So far two patients completed the course of the study without severe side effects except for fever and skin rash, and their clinical outcomes were partial remission and stable disease respectively.

Authors’ Affiliations

(1)
Department of Hematology, National Kyushu Cancer Center
(2)
Department of Immunotherapeutics, Tokyo Medical and Dental University
(3)
Department of Medicine and Biosystemic Science, Kyushu University
(4)
Institute of Medical Science, University of Tokyo
(5)
Department of Hematology, Tokyo Medical and Dental University
(6)
Center for Advanced Medicine Innovation, Kyushu University
(7)
Clinical Laboratory Division, National Cancer Center Hospital
(8)
Department of Hematology, Imamura Bun-in Hospital
(9)
Institute for Virus Research, Kyoto University
(10)
Department of Hematology, Hokkaido University
(11)
Institute for Clinical Research, National Kyushu Cancer Center

Copyright

© Suehiro et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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