Figure 7
The production of virions after infecting Cf2Th cells. The cells that were 70-80% confluent were grown in 6 well plates and then infected with equal amounts (2.5 × 105 viral particles per infection) of EIAV and of the different BIV variants (WT, D48E/N57S, Δ36)- all produced by transfection (see Figure 5). All infections were performed in the presence of 10 μg/ml DEAE-Dextran. The titers of the viral particles produced 4–6 weeks post-infection were determined, as described in Methods. The presented data were taken 5 weeks after infection (namely, 8 cell passages), as described in Methods.