Table 1 Genes differing by 2-fold or more expression between Ag and Ag + Ant treatment

FOS

5.58

Immediate early gene transcription factor, up-regulated in response to extracellular signals like chemokines and cytokines

CCL3L1

5.07

Codes for protein (Mip-1α) that binds CCR5 and caused internalization of CCR5, preventing HIV entry

[34, 35]

IL8

4.81

Inflammatory cytokine produced by T cells, cell chemoattractant

EGR2

4.75

Early growth response protein, responsible for regulation cell activation, overexpression leads to inhibition of T cell activation

[36]

NR4A2

4.62

Involved in T cell homeostasis

NR4A3

4.33

Involved in T cell homeostasis

DUSP2

3.83

Phosphatase that inactivate MAP kinase cascade, negatively regulate proliferation and differentiation

[37, 38]

CSF2

3.49

Cell growth factor

TNFSF9

3.26

TNF superfamily trans-membrane glycoprotein expressed on activated T cells

MAP3K8

2.97

Activates MAP and JNK kinase pathways and induces NF-κB, promotes TNF-α and IL-2 during T cell activation

[39, 40]

TNF

2.96

Pro-inflammatory cytokine

CD69

2.94

Early inducible surface glycoprotein, functions as signal transducing receptor

EGR1

2.85

Early growth response protein, enhances T cell function

[36]

SPRY1

2.77

Inhibits TCR signaling in differentiated cells, enhances TCR signaling in naïve cells

[41]

IFNG

2.71

Pro-inflammatory cytokine, antiviral and immunoregulatory functions

TAGAP

2.63

Rho GTPase activating protein, role in T cell activation

FOSB

2.62

Proteins that dimerize with JUN, form AP-1 transcription complex, role in proliferation and differentiation

[42]

CCL3

2.48

Pro-inflammatory cytokine (MIP-1α), natural ligand for CCR5

[43]

BTG2

2.47

Negative regulator of cell cycle

[44]

GADD45B

2.44

Increased levels observed following cell stress, inhibit cell growth

[45]

CCL3L3

2.44

Cytokine involved in immunoregulatory and inflammatory process, can bind to CCR5, inhibits HIV entry

[34, 35]

NR4A1

2.27

Can block activation through NF-κB,

[46]

PDCD1

2.23

Negatively regulates TCR signals

[47]

EGR4

2.17

Interacts with NF-κB, controls transcription of genes encoding inflammatory cytokines including IL-2

[48]

ZFP36

2.1

Binds to 3’-UTR of some cytokines and promotes their degradation

[49]

PTGS2

2.09

Cox-2 has been shown to be Involved in Th1 differentiation

NFKBID

2.03

Member of NF-κB inhibitor family

FASLG

2.02

FAS-FASL binding is involved in T-cell homeostasis

FAM53C

2.00

Unknown function

GJB2

-2.13

Gap junction beta-2 protein

HBB

-2.20

Haemoglobin beta subunit

SPDEY9P

-2.25

Speedy/RINGO cell cycle regulator family member E9

CCR7

-2.28

G-protein coupled family receptor involved in T cell migration