Figure 6

Induction of p53 ex vivo in 9AA and 9AA with Campath-1H treated MET-1 cells. (A) Western blot analysis of expression levels of p53, phospho-p65, p65, and PARP in splenocytes from MET-1 injected mice. Thirteen days after therapy, splenic cells from MET-1 tumor-bearing mice were separated. Cell lysates were immunoblotted with the indicated antibodies: PARP, p53, phospho-p65 and p65. α-Tubulin was used as an input control. (B) p53 responsive genes increased after 9AA and 9AA plus Campath-1H treatment. Total cellular RNA was isolated from splenic cells of mice from each treatment group. RT-PCR was performed and the levels of hdm2 and bax genes were measured. The levels of hdm2 and bax for control animals were set at 1 and the graph represents results from three independent experiments.