Figure 5

9AA combined with Campath-1H significantly prolonged survival of MET-1 leukemia-bearing mice. (A) On day 1, the serum levels of sIL-2Rα for the four groups ranged from 3,189 to 3,486 pg/mL. Two weeks after therapy, the serum values rose to 72,737 pg/mL for control mice and 32, 654 pg/ml for 9AA mice (9AA vs control, p > .05). The sIL-2Rα levels for Campath-1H (Campath-1) treated mice showed no increase compared to initial levels, 3,270 pg/mL (Campath-1H vs control, p < .01). The combination group decreased to 1,810 pg/mL (combination vs control, or vs 9AA, p < .01). Four weeks after therapy, sIL-2Rα was 279,302 pg/mL and 102,233 pg/mL for control and 9AA groups, respectively (p < .01). Serum sIL-2Rα for the Campath-1H group increased to 7,674 pg/mL (Campath-1H vs control, p < .001). The combination group remained at 1,330 pg/mL (combination vs control, or vs 9AA, p < .001). (B) On day 1, the serum levels of β2μ for the four groups were less than 0.05 μg/mL. Four weeks after therapy, the serum β2μ values of the control and 9AA groups were 9.25 μg/mL and 5.0 μg/mL, respectively (p > .05). The serum β2μ values of the Campath-1H group increased to 0.13 μg/mL (Campath-1H vs control, p < .0001). The serum β2μ values were below detection for the combination group (combination vs control, or vs 9AA, p < .0001). (C) Kaplan-Meier analysis demonstrating combination therapy of 9AA and Campath-1H prolonged survival of MET-1 leukemia-bearing mice. (p < .0001). (D) Four of 8 mice in the Campath-1H group and 8 of 8 mice in the combination group survived to 100 days. The sIL-2Rα levels for the groups of Campath-1H and the combination were 296,467 pg/mL and 4,609 pg/mL, respectively (p < .001).