Figure 2

Increased active caspase-3 and cleaved PARP in ATL cell lines after 9AA treatment. Jurkat, MT-1, ED40515 (-), 43 Tb (-), ED40515 (+), and LM-Y1 cells were treated for 48 hours with and without 9AA at 2 μM, or 5 μM. The cells were stained with anti-cleaved PARP and anti-active caspase3, and then analyzed by FACS for apoptotic cells with active caspase-3 and cleaved PARP. Data are representative of 3 independent experiments.