Figure 3

Kinetics of 447-52D antibody binding to gp140 constructs with SPR measurements. ProteOn XPR36 measurements were performed with immobilized 447-52D antibody (ligand) and rising concentrations of the different gp140 constructs (analyte). Note the remarkable slower dissociation of the NL4-3/ADA construct from the 447-52D mAb in contrast to the NL4-3 and ADA construct, which is responsible for its higher affinity to 447-52D (see also Figure 2 and table of Additional file 6 with listed K_D, k_on and k_off rates).