Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunits

Table 1 Neutralizing and binding properties of bNAbs: stoichiometry, off-rate constant, and persistent fraction (PF)

NAb	S _m	k_off1(1/s)	Log PF
VRC01 (n = 6)^a	1.6	4.7 ^. 10⁻⁶	−3.7 ± 0.80
PGV04 (n = 4)	1.5	<1.0 ^. 10⁻⁵	−2.5 ± 0.17
PGT121 (n = 10)	1.7	8.5 ^. 10⁻⁶	−2.8 ± 0.089
PGT122 (n = 8)	1.6	3.0 ^. 10⁻⁶	−3.2 ± 0.39
PGT123 (n = 8)	2.0	9.5 ^. 10⁻⁶	−3.1 ± 0.14
2G12 (n = 6)	2.9	1.5 ^. 10⁻⁴	−1.2 ± 0.082
PG9 (n = 6)	0.97	7.1 ^. 10⁻⁴	−2.1 ± 0.12
PG16 (n = 8)	0.96	2.7 ^. 10⁻³	−1.6 ± 0.071
PGT145 (n = 6)	0.78	6.9 ^. 10⁻⁴	−1.3 ± 0.12
PGT151 (n = 6)	1.8	3.5 ^. 10⁻⁴	−3.0 ± 0.086

^aThe values in the Table are derived from global non-linear regression fits of data from n replicate neutralization titrations ± s.e.m. for the logarithmic PF values. The n values for SPR analyses are given in Additional file 3: Tables S4 and S5, where the S_mand k_off1 are included ± s.e.m.

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