Interaction affinity of SC29EK and MT-SC29EK with N36 measured by ITC assay. 1 mM N36 dissolved in double distilled H2O was injected into the chamber containing 100 μM SC29EK (Left) or MT-SC29EK (Right). The experiments were carried out at 25°C. Data acquisition and analysis were performed using MicroCal Origin software (version 7.0). The upper panels show the titration traces and the lower panels show the binding affinity when the N36 solution was injected into the SC29EK or MT-SC29EK solution.