The association between the two overlapping epitopes and the Y135F mutation. (A) HIV-1 map of representative proteins and the locations of HLA-A*24:02-restricted epitopes used in this study are shown (arrow). The positional relation and the sequence information around Nef126-10 and Nef134-10 epitopes are also shown. (B) Immune responses to 11 epitopes were assessed by IFN-γ ELISpot assay by using expanded PBMCs from 46 HIV-1 patients. Epitope names below the chart indicate peptides used in the assay; [Protein][Location(HXB2 numbering)]-[Amino acid length]. Each point represents the average SFU of duplicate wells after subtraction of background in each individual. Each stick represents response rate in 46 individuals. (C) The Nef126-10/Nef134-10-specific response rates among individuals harboring either Nef135Y or Nef135F were assessed. Black bars indicate number of responders to each epitope, and white bars for non-responders.