Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site

Table 7 The infectivity of AE-Env clones containing D185 and the derived mutants

^aInfectivity of Env-recombinant virus was evaluated using U87.CD4.CXCR4 and U87.CD4.CCR5 cells. Relative infectivity of the virus was calculated by comparing it with the luciferase activity of pNL-envCT (pNL4-3)-infected U87.CD4.CXCR4 cells, which was defined as 100 relative light units (RLU).
^bSingle or multiple amino acid mutations were introduced into AE-Env-recombinant viruses. Amino acid numbering is based on HXB2 Env gp120.

ISSN: 1742-4690