Figure 5

Chromatin and DNA methylation assessment of the High, Medium, and Negative expressing sorted cells. (A) ChIP–based measurement of H3K9me3, (B) H3K27me3 and (C) H3K4me3 at the viral U5-PBS region, ERV class I (mlvGLN) and ERV class II (IAPpol) in sorted populations. Enrichment values are relative to total input and normalized to the signal of negative control (Gapdh or Necap1). (D) Bisulfite sequencing analysis of the 5′LTR of the infecting virus was conducted on genomic DNA isolated from the sorted cells. Open and filled circles represent unmethylated or methylated cytosines, respectively. (E) Bisulfite sequencing analysis of Oct4 was used as control.