Figure 4

After a second sort, a subpopulation of stable expressing cells can be isolated and characterized. (A) Flow analysis of cells infected with PBSpro virus, first sorted as double positive, and then monitored on the day of the second sorting. Infected (white) and uninfected control cells (black), and gate names, are indicated. (B-D) Flow analysis for 30 days after second FACS sorting of cells from (B) High expressing- double positive cells. (C) Medium expressing- double positive cells after second sorting. (D) mCherry-only positive cell and (E) GFP only positive cells. On the right – an example of the flow analysis dot plot as recorded 26 days after second sorting.