Figure 2

ISG modulation by Tat alleles and mutants in iDC. A. RNA expression analysis in iDCs expressing Tat alleles and mutants. Gene expression fold induction is shown for RNA isolated 10 h after adenovirus infection or interferon treatment, and 10 and 14 days after HIV-1 infection. Data for HIV-1 infection, previously published [23], are included here for direct comparison. GAPDH levels were used to normalize the results of the individual samples. B. Microarray mRNA levels of selected ISG in iDC expressing Tat alleles and mutants or exposed to IFNs (10 h time point). RNA fold change is reported as the ratio of the signal in the experimental sample compared to the control. C. Gene expression analysis of selected ISG RNA levels by RT-PCR (upper panel) and protein levels by ELISA (lower panel, 20 h post-infection) in cell lysates and their respective supernatants of iDC from five donors. RNA fold change compared to that of control cells infected with Ad-tTA, was evaluated using the threshold cycle (Ct) number normalized for β-actin Ct.