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Figure 6 | Retrovirology

Figure 6

From: Nuclear import of SAMHD1 is mediated by a classical karyopherin α/β1 dependent pathway and confers sensitivity to VpxMAC induced ubiquitination and proteasomal degradation

Figure 6

The nuclear fraction of SAMHD1 K11A in MDM is sensitive to Vpx MAC induced degradation. A) Monocyte-derived macrophages were transduced with lentiviral vectors encoding HA-tagged SAMHD1WT or SAMHD1K11A in the presence of virus like particles (VLPs) delivering VpxMAC or control VLPs and cells were fixed for HA-immunostaining 72 h later. Scale bars, 30 μm. Shown are representative pictures from one of four independent donors. B) The integrated MFI densities were calculated on MDM expressing HA-SAMHD1WT or HA-SAMHD1K11A for nuclear and cytoplasmic areas using ImageJ software. Numbers within the graphs show fold changes of the average integrated densities normalized to control VLP treated cells.

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Retrovirology

ISSN: 1742-4690

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